FisMat2017 - Submission - View

Abstract's title: Study of nucleic acids extracted from tumor cells by means of UV Resonant Raman and FTIR spectroscopies
Submitting author: Paolo Zucchiatti
Affiliation: University of Trieste, Elettra-Sincrotrone Trieste, Istituto Italiano di Tecnologia
Affiliation Address: Dipartimento di Fisica, Università degli Studi di Trieste, Via Alfonso Valerio, 2, 34127 Trieste, Italy - Elettra Sincrotrone Trieste, S.S. 14 Km 163.5 34149 Trieste, Italy - Istituto Italiano di Tecnologia, IIT, Via Morego, 30 16163 Genova, Italy
Country: Italy
Oral presentation/Poster (Author's request): Poster
Other authors and affiliations: Paolo Zucchiatti (Universitò di Trieste, Elettra Sincrotrone Trieste, IIT Genova), Katia Latella (Università di Genova), Lisa Vaccari (Elettra Sincrotrone Trieste), Barbara Rossi (Elettra Sincrotrone Trieste) and Francesco D’Amico (Elettra Sincrotrone Trieste)

Vibrational spectroscopies have been widely employed to investigate the nucleic acids structure. Particularly, Raman spectroscopy has been used in the past to characterize the chemical structure of DNA by using UV excitation instead of visible one, allowing to obtain a higher scattering efficiency without intense fluorescence backgrounds [1]. FTIR spectroscopy, instead, offers many distinct bands indicative of the major DNA and RNA conformation and it allows distinguishing the nucleic acids structural changes upon environmental modifications, mainly according to the intensity and the position of the bands relative to the phosphodiester bonds [2]. Spectra of the two techniques are complementary each other. We carried out a series of Raman and FTIR measures, performed in parallel at the IUVS and SISSI beamlines at Elettra. The aim of these experiments was to collect FTIR and Raman spectra from both DNA and RNA extracted from cells. We compared Raman spectra acquired with different excitation wavelength, with a spectrum acquired with single reflection ATR-FTIR spectroscopy. The results highlighted the range where Raman shifts are relevant, and mostly associated to Adenine and Guanine. It was evident that the main signals for FTIR spectroscopy fell outside this range. In the 1500-1280 cm-1 spectral region there are indeed infrared signals, which however are very weak. We believe that by exploiting the complementarity of the two vibrational techniques we can achieve a complete overview of the biochemistry of this molecular system.


[1]    F. D’Amico, and L. Pascolo, Analys, Rcs, 140 (2015).
[2]    B. R. Wood, Chem. Soc. Rev., Acs, 45 (2016).