FisMat2017 - Submission - View

Abstract's title: Amyloid β peptides aggregation and their interaction with raft-mime model membranes
Submitting author: Valeria Rondelli
Affiliation: Università degli Studi di Milano
Affiliation Address: Dipartimento di Biotecnologie Mediche e Medicina Traslazionale polo LITA Via F.lli Cervi 93, Segrate
Country: Italy
Oral presentation/Poster (Author's request): Oral presentation
Other authors and affiliations: Paola Brocca (Università degli Studi di Milano), Giovanna Fragneto(ILL), Elena Del Favero(Università degli Studi di Milano), Laura Cantù(Università degli Studi di Milano)

A hallmark pathological feature of Alzheimer’s disease (AD) is aggregation and deposition of β-Amyloid peptides (Aβ) in the brain. Different complementary techniques have been applied to study the aggregation of different Aβ peptides, from monomers to fibrils, and their interaction with model membranes with biomimetic composition. Laser light scattering has been applied to investigate the early stages of Aβ peptides aggregation. Calorimetry and X-ray and neutron scattering have been used to investigate the effect of Aβ peptide interaction on the thermotropic and structural behavior of model membranes in  bulk, while neutron reflectivity has been applied to study, with the Angstrom sensitivity, the structural interaction of peptides with complex membranes prepared by the Langmuir-Blodgett deposition technique [1]. Peptide-membrane interaction was found to depend both on membrane composition and on the state of aggregation of the peptide.Moreover the N-terminal portion of Aβ was seen to interact with lipids of the bilayer probably promoting the penetration of peptide in the membrane.


[1] V. Rondelli, P. Brocca, S. Motta, M. Messa, L. Colombo, M. Salmona, G. Fragneto, L. Cantù, E. Del Favero, Sci. Rep. , 2016, 6, 20997.