FisMat2017 - Submission - View

Abstract's title: Electrochemical Biosensor for PCR free Nucleic Acids Detection
Submitting author: Sabrina Conoci
Affiliation: STMicroelectronics
Affiliation Address: Stradale Primosole 50, 95121 Catania
Country: Italy
Oral presentation/Poster (Author's request): Poster
Other authors and affiliations: Salvatore Petralia1, ML Di Pietro2, Emanuele L. Sciuto3 . Massimo Zimbone3, Maria. G. Grimaldi3 and Sabrina Conoci1 1STMicroelectronics ADG Central R&D Catania, Stradale Primosole, 50 Italy 2 Dept. Chemical Science, Univ. of Messina. V.le F. Stagno d'Alcontres, 31 I-98166 Messina 3Diaprtimento di Fisica e Astronomia, Università diCatania, Via Santa Sofia 64, 95123 Catania.
Abstract

An effective chemical modification startegy, integrated in a silicon biosensor, was investigated for the rapid and sensitive electrochemical detection of low amount of pathogen DNA without the amplification PCR step. The miniaturised biosensor was composed by three planar microelectrodes for the electrochemical transduction for the hybridization event with DNA target occurring at the working electrode. The event is proved by an intercalative Os-complex. The core of the proposed detection strategy is based on the immobilization on the working electrode of two oligonucleotide probes, specific for the two DNA semi-elix. The assay here presented was able to detect up to 200 copies of HBV (Hepatites Virus) genome extracted from human blood.

REFERENCES

Petralia et al, Analyst 2017, Advanced article DOI:10.1039/C7AN00202e

Petrali et al, Sensing and Biosensing Research 2015, 6, 690-694